GPX4 antibody (C-Term)

Catalog No. ABIN401375

The Rabbit Polyclonal anti-GPX4 antibody has been validated for WB and EIA. It is suitable to detect GPX4 in samples from Human, Mouse, Rat and Guinea Pig.

Quick Overview for GPX4 antibody (C-Term) (ABIN401375)

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Reactivity: Human, Mouse, Rat, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GPX4 Antibody

Binding Specificity: C-Term

Specificity: This antibody is directed against GPx4 protein.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Guinea Pig, Human.
Species reactivity (tested):Mouse.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/7,000 - 1/30,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.15 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: Glutathione Peroxidase 4 / GPX4

Background: Glutathione Peroxidase 4 (GPx4, also known as PHGPx) functions as an antioxidant and also plays a role in redox regulation, sexual maturation, inflammation, apoptosis, and differentiation. GPx4 was found to have an important role in preventing lipid peroxidation, and has been discussed primarily as the glutathione peroxidase protecting biomembranes against oxidative stress. GPx4 can react with free hydrogen peroxide as well as with a wide range of lipid hydroperoxides, including those derived from cholesterol and cholesteryl esters. GPx4 is the only GPx to use phospholipid hydroperoxides as substrates. It can also reduce thymine hydroperoxide. In contrast to GPx1 and GPx3, GPx4 can use a wide range of reducing substrates in addition to glutathione. GPx4 is expressed in high levels in testes, suggesting a role in male fertility. It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm.Synonyms: GPx-4, Phospholipid hydroperoxide glutathione peroxidase mitochondrial

Gene ID: 2879

NCBI Accession: NP_001034936

UniProt: P36969