AHSA1 antibody (Internal Region)

Catalog No. ABIN401377

This Rabbit Polyclonal antibody specifically detects AHSA1 in WB and EIA. It exhibits reactivity toward Human and Chimpanzee.

Quick Overview for AHSA1 antibody (Internal Region) (ABIN401377)

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AHSA1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-AHSA1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody is directed against AHA1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Chimpanzee.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human AHA1 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/35,000 - 1/185,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.08 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for AHSA1

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Alternative Name: AHA1 / AHSA1

Background: Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 Molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist clientprotein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.Synonyms: Activator of 90 kDa heat shock protein ATPase homolog 1, C14orf3, HSPC322

Gene ID: 10598

NCBI Accession: NP_036243

UniProt: O95433