SUMF1 antibody (Internal Region)

Catalog No. ABIN401525

The Goat Polyclonal anti-SUMF1 antibody has been validated for WB and EIA. It is suitable to detect SUMF1 in samples from Human, Mouse, Rat, Cow and Dog.

Quick Overview for SUMF1 antibody (Internal Region) (ABIN401525)

Target: SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This SUMF1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SUMF1 Antibody

Binding Specificity: Internal Region

Sequence: c-ETLNPKGPPS GKDR

Specificity: This antibody detects SUMF1 / FGE (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Dog, CowSpecies reactivity (tested):Human, Mouse

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_877437.2 . Genename: SUMF1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 0.5-2 μg/mL. Approx 37-40 kDa band observed in Mouse Pancreas, Mouse Eyeand Human Kidney lysates (calculated MW of 40.0 kDa according to NP_877437.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for SUMF1

Target: SUMF1 (Sulfatase Modifying Factor 1 (SUMF1))

Alternative Name: SUMF1 / FGE

Background: SUMF1 is a sulfatase modifying enzyme that oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD). MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive.Synonyms: C-alpha-formylglycine-generating enzyme 1, Sulfatase-modifying factor 1

Gene ID: 285362

NCBI Accession: NP_877437

UniProt: Q8NBK3