CPI-17 antibody (Isoform alpha, Thr38)

Catalog No. ABIN401630

Product Details anti-CPI-17 Antibody

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Binding Specificity: Isoform alpha, Thr38

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPI-17 antibody is un-conjugated

Application: Immunofluorescence (IF)

Specificity: CPI17α antibody detects endogenous levels of total CPI17α protein.

Purification: Affinity chromatography

Immunogen: Synthesized non-phosphopeptide derived from human CPI17alpha around the phosphorylation site of threonine 38 (R-V-TP-V-K)

Application Details

Application Notes: Immunofluorescence: 1/100 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CPI-17

Target: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Alternative Name: PPP1R14A (PPP1R14A Products)

Background: PKC potentiated inhibitor protein-17 is a 17 kDa protein. Expressed in smooth muscles and neuronal cells, CP117 is a myosin phosphatase inhibitor protein. Phosphorylation of CP117 at Thr38 enhances its inhibitory effect by 100 fold. Once phosphorylated, CP117 inhibits PP1c and MLCP holoenzyme activity.Synonyms: 17 kDa PKC-potentiated inhibitory protein of PP1, CPI-17, CPI17, PPP1INL, Protein phosphatase 1 regulatory subunit 14A

Gene ID: 94274

NCBI Accession: NP_150281

UniProt: Q96A00