FZD8 antibody (Cysteine-Rich Domain, Extracellular)

Catalog No. ABIN410246

The Goat Polyclonal anti-FZD8 antibody has been validated for WB, ELISA and IHC. It is suitable to detect FZD8 in samples from Mouse.

Quick Overview for FZD8 antibody (Cysteine-Rich Domain, Extracellular) (ABIN410246)

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-FZD8 Antibody

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-8 extracellular Cysteine-Rich Domain (CRD). Approximately 5 % cross-reactivity with rmFrizzled-9 and less than 1 % cross-reactivity with rmFrizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-4, rmFrizzled-6 and rmFrizzled-7.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmFrizzled-8 extracellular Cysteine-Rich Domain (CRD).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 - 15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Direct ELISA, Western blot, Immunohistochemistry. Direct ELISA: 0.5-1 μg/mL. Western blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-8. The detection limit for rmFrizzled-8 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 10-15 μg/mL in fixed cells or tissues.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 1 mL of Sterile PBS.

Concentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FZD8

Target: FZD8 (Frizzled Family Receptor 8 (FZD8))

Alternative Name: FZD8 / Frizzled 8

Background: Name/Gene ID: FZD8
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD8, Frizzled family receptor 8, Frizzled homolog 8, FZ-8, Fz8, Frizzled 8, Frizzled-8, HFZ8

Gene ID: 8325

UniProt: Q9H461

Pathways: WNT Signaling