Aflatoxin antibody

Catalog No. ABIN452582

The Rat Monoclonal anti-Aflatoxin antibody has been validated for LF and EIA. It is suitable to detect Aflatoxin in samples from Aspergillus.

Quick Overview for Aflatoxin antibody (ABIN452582)

Target: Aflatoxin (AFT)

Reactivity: Aspergillus

Host: Rat

Clonality: Monoclonal

Conjugate: This Aflatoxin antibody is un-conjugated

Application: Lateral Flow (LF), Enzyme Immunoassay (EIA)

Clone: 2-00E-004

Product Details anti-Aflatoxin Antibody

Purification: Affinity Chromatography on Protein G.

Immunogen: Winstar rats were immunized with Aflatoxin M1-BSA conjugate.

Isotype: IgG2b

Application Details

Application Notes: ELISA: 100 ng of AFM1 can be detected as competitive conjugate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2 without preservatives

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody in aliquots at -20 °C after reconstitution. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Aflatoxin

Target: Aflatoxin (AFT)

Alternative Name: Aflatoxin

Target Type: Chemical

Background: The aflatoxins are a group of closely related mycotoxins that are widely distributed in nature. The most important of the group is aflatoxin B1 (AFB1), which has a range of biological activities, including acute toxicity, teratogenicity, mutagenicity and carcinogenicity. In order for AFB1 to exert its effects, it must be converted to its reactive epoxide by the action of the mixed function mono-oxygenase enzyme systems (cytochrome P450-dependent) in the tissues (in particular, the liver) of the affected animal. This epoxide is highly reactive and can form derivatives with several cellular macromolecules, including DNA, RNA and protein. Cytochrome P450 enzymes may additionally catalyse the hydroxylation (to AFQ1 and AFM1) and demethylation (to AFP1) of the parent AFB1?Molecule, resulting in products less toxic than AFB1. Conjugation of AFB1 to glutathione (mediated by glutathione S-transferase) and its subsequent excretion is regarded as an important detoxification pathway in animals.