HSF2 antibody

Catalog No. ABIN452666

The Rat Monoclonal anti-HSF2 antibody has been validated for WB, IF, IP, IHC (fro), EIA and EMSA. It is suitable to detect HSF2 in samples from Various Species.

Quick Overview for HSF2 antibody (ABIN452666)

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Reactivity: Various Species

Host: Rat

Clonality: Monoclonal

Conjugate: This HSF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: 3-00E-002

Product Details anti-HSF2 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit, Dog, Bovine, Sheep, Pig.

Characteristics: Synonyms: HSF-2, HSTF2, Heat shock transcription factor 2

Purification: Purified

Immunogen: Purified recombinant mouse SF2 protein

Isotype: IgG1

Application Details

Application Notes: Western blot: 4 μg/mL was sufficient for detection of HSF2 of HeLa lysate. Gel Shift Assay (EMSA). Immunoprecipitation. Immunoflourescence. Immunohistochemistry on frozen sections. ELISA.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2, 0.09 % Sodium Azide, 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HSF2

Target: HSF2 (Heat shock factor protein 2 (HSF2))

Alternative Name: Heat Shock Factor 2 / HSF2

Background: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).Synonyms: HSF-2, HSTF2, Heat shock transcription factor 2

UniProt: P38533