BCKDHA antibody (C-Term)

Catalog No. ABIN452780

The Rabbit Polyclonal anti-BCKDHA antibody has been validated for WB and EIA. It is suitable to detect BCKDHA in samples from Human.

Quick Overview for BCKDHA antibody (C-Term) (ABIN452780)

Target: BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCKDHA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BCKDHA Antibody

Binding Specificity: C-Term

Specificity: This antibody detects BCKDHA at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: KLH conjugated synthetic peptide selected from the C-terminal region of human BCKDHA

Application Details

Application Notes: Western blot: 1: 50 - 1: 100. ELISA: 1: 1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.123 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for BCKDHA

Target: BCKDHA (Branched Chain Keto Acid Dehydrogenase E1, alpha Polypeptide (BCKDHA))

Alternative Name: BCKDHA

Background: The branched-chain alpha-keto acid (BCAA) dehydrogenase(BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). BCKDHA is the alpha subunit of the decarboxylase (E1) component.Synonyms: 2-oxoisovalerate dehydrogenase subunit alpha mitochondrial, BCKDE1A, Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, alpha-keto acid dehydrogenase

Molecular Weight: 50471 Da

Gene ID: 593

NCBI Accession: NP_000700

UniProt: P12694