PLA2G4A antibody (Middle Region)

Catalog No. ABIN453281

The Rabbit Polyclonal anti-PLA2G4A antibody has been validated for WB and EIA. It is suitable to detect PLA2G4A in samples from Human.

Quick Overview for PLA2G4A antibody (Middle Region) (ABIN453281)

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLA2G4A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PLA2G4A Antibody

Binding Specificity: Middle Region

Specificity: This antibody reacts to PLA2G4A.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide selected from the Center region of human PLA2G4A

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PLA2G4A

Target: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Alternative Name: PLA2G4A

Background: PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.Synonyms: Cytosolic phospholipase A2, Phospholipase A2 group IVA, cPLA2, phospholipase A2

Gene ID: 5321

NCBI Accession: NP_077734

UniProt: P47712

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling