Mouse anti-Human IgD Antibody (FITC)

Catalog No. ABIN457874

Quick Overview for Mouse anti-Human IgD Antibody (FITC) (ABIN457874)

Target: IgD

Clonality: Monoclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Clone: NI 158

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to a isotype specific determinant of the IgD molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgD, isotype specific

Characteristics: Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgD, isotype specific
Fluorochrome/IgG protein molar ratio (E/P) approximately 1.7.

Purification: Purified

Immunogen: Highly purified IgD isolated from human serum.

Application Details

Application Notes: To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgD and cell membrane IgD.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized prod uct by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgD

Target Type: Antibody