Mouse anti-Human IgD Antibody (FITC)
IF
Monoclonal
NI 158
IgG1 kappa
FITC
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- Target See all IgD products
- IgD
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- FITC
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Application
- Immunofluorescence (IF)
- Specificity
- The reactivity of the antiserum is restricted to a isotype specific determinant of the IgD molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgD, isotype specific
- Characteristics
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Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgD, isotype specific
Fluorochrome/IgG protein molar ratio (E/P) approximately 1.7. - Purification
- Purified
- Immunogen
- Highly purified IgD isolated from human serum.
- Clone
- NI 158
- Isotype
- IgG1 kappa
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- Application Notes
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To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgD and cell membrane IgD.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only. - Comment
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Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute the lyophilized prod uct by adding 0. 5 mL sterile distilled water.
- Concentration
- 0.4 mg/mL
- Buffer
- Purified monoclonal mouse IgG1 kappa conjugated with fluorescein isothiocyanate, lyophilized from a solution in phosphate buffered saline (pH 7.2).
- Preservative
- Without preservative
- Handling Advice
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- IgD
- Abstract
- IgD Products
- Target Type
- Antibody
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