SERPINA1 antibody

Catalog No. ABIN457941

Product Details anti-SERPINA1 Antibody

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SERPINA1 antibody is un-conjugated

Application: Immunoelectrophoresis (IEP), Immunodiffusion (ID), Radial Immunodiffusion (RID)

Specificity: The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction has been found with Rhesus monkey, Cynomolgus, Baboon and Chimpanzee, no reaction with mouse.

Characteristics: Precipitating polyclonal goat antiserum to human alpha-1 antitrypsin

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Alpha-1 antitrypsin is a serine protease inhibitor. It has a molecular weight of 54,000, its mean concentration in normal serum is 2.5 mg/ml. The highly purified immunogen is isolated from pooled normal plasma. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of alpha-1antitrypsin in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against in agar-block immunodiffusion titration against an appropriate

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C

Target Details for SERPINA1

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Alternative Name: alpha-1 Antitrypsin (SERPINA1 Products)

Background: The reactivity of the antiserum is restricted to alpha-1antitrypsin. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified alpha-1antitrypsin. No reaction is obtained with any other plasma protein component or serum