IgA Secretory Component antibody (FITC)

Catalog No. ABIN458014

This Goat Polyclonal antibody specifically detects in ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for IgA Secretory Component antibody (FITC) (ABIN458014)

Target: IgA Secretory Component

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: FITC

Application: ELISA, Immunofluorescence (IF)

Product Details

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to human secretory component

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity to any other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Immunogen: Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM. Purified free human secretory component isolated from pooled milk is used for immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: As reagent for the direct detection of secretory component in human cells, tissues and body fluids in immuno-fluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Fluorescein/IgG protein molar ratio (F/P) approximately 1.6. No foreign proteins added.

Buffer: Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Storage: 4 °C

Target Details

Target: IgA Secretory Component

Alternative Name: Secretory Immunoglobulin A (SIgA)

Background: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alpha-2 region. The immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein