LYZ antibody

Catalog No. ABIN458107

Product Details anti-LYZ Antibody

Target: LYZ (Lysozyme (LYZ))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LYZ antibody is un-conjugated

Application: Immunocytochemistry (ICC), Immunodiffusion (ID), Radial Immunodiffusion (RID), Immunoelectrophoresis (IEP)

Specificity: The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal rabbit antiserum to human lysozyme

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.

Immunogen: Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low, in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme. Highly purified lysozyme is isolated from pooled milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure lysozyme. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Physicochemical characteristic IgG protein concentration 10 mg/ml. No foreign proteins added. Antibody titre: Precipitin titre not les than 1:8 when tested against pooled normal human milk in agar block immunodiffusion titration.

Buffer: Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2)

Preservative: Without preservative

Storage: 4 °C

Target Details for LYZ

Target: LYZ (Lysozyme (LYZ))

Alternative Name: Lysozyme (LYZ Products)

Background: The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum