PF4 antibody

Catalog No. ABIN458158

This Rabbit Polyclonal antibody specifically detects PF4 in ELISA, IEP, RID, Neph and ID. It exhibits reactivity toward Human.

Quick Overview for PF4 antibody (ABIN458158)

Target: PF4 (Platelet Factor 4 (PF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: ELISA, Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID), Nephelometry (Neph), Immunodiffusion (ID)

Product Details anti-PF4 Antibody

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail. In double radial immunodiffusion a reaction has been observed with bovine, cat, dog and monkey.

Characteristics: Precipitating polyclonal rabbit antiserum to human platelet factor 4

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Platelet factor 4 is a low molecular weight (MW 7,800) heat stable protein, secreted by alpha granules of the platelets. Purified platelet factor 4 isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as electroimmunodiffusion (Laurell), immunoelectrophoresis and single and double radial immunodiffusion to identify the presence platelet factor 4. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included. Measurement of platelet factor 4 is of significance for diagnosis of platelet disorders involving platelet alpha granules and to demonstrate increased platelet secretion in vivo. Reduced levels are measured in gray platelet syndrome, whereas elevated levels have been reported in cases as coronary artery disease, myocardial infarction, disseminated intravascular coagulation, cerebrovascular disease and cancer.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: The amount of platelet factor 4 precipitated by 1 ml antiserum is approximately 0.1 mg.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: Platelet Factor 4

Background: The reactivity of the antiserum is restricted to platelet factor 4 as tested in immunoelectro-phoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified immunogen