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Erythrocytes antibody

The Rabbit Polyclonal anti- antibody has been validated for ScA. It is suitable to detect in samples from Sheep.
Catalog No. ABIN458771

Quick Overview for Erythrocytes antibody (ABIN458771)

Target

Erythrocytes

Reactivity

  • 13
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 1
Sheep

Host

  • 26
  • 14
Rabbit

Clonality

  • 26
  • 14
Polyclonal

Conjugate

  • 19
  • 6
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Un-conjugated

Application

  • 14
  • 13
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Screening Assay (ScA)
  • Specificity

    Inter-species cross-reactivity is a normal feature of antibodies since different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

    Characteristics

    Polyclonal rabbit antiserum to sheep erythrocytes

    Purification

    Adsorption: No adsorption required. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies since different species frequently share antigenic determinants.

    Immunogen

    Washed whole erythrocytes were prepared from fresh blood and used for intravenous immunization. Freund’s complete adjuvant is used in the first step of the immunization procedure.

    Isotype

    IgG
  • Application Notes

    To sensitize cells in hemolytic complement assays and for lymphocytic screening procedures.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Concentration

    Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Hemolytic titre at which 50% lysis occurs following mixing of equal volumes of antiserum, guinea pig co

    Buffer

    Delipidated, heat inactivated, lyophilized, stable whole antiserum

    Preservative

    Without preservative

    Storage

    4 °C/-20 °C

    Storage Comment

    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
  • Target

    Erythrocytes
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