Lactat Dehydrogenase (M4) (LDH-5) antibody (Biotin)

Catalog No. ABIN459128

The Sheep Polyclonal anti- antibody has been validated for IF, ELISA and WB. It is suitable to detect in samples from Rabbit.

Quick Overview for Lactat Dehydrogenase (M4) (LDH-5) antibody (Biotin) (ABIN459128)

Target: Lactat Dehydrogenase (M4) (LDH-5)

Reactivity: Rabbit

Host: Sheep

Clonality: Polyclonal

Conjugate: Biotin

Application: Immunofluorescence (IF), ELISA, Western Blotting (WB)

Product Details

Specificity: Cross-reactivities against enzymes of other sources may occur but have not been determined.

Characteristics: Biotin-conjugated IgG fraction of polyclonal sheep antiserum to L-lactic dehydrogenase, LDH-5(M 4 ) isoenzyme, from rabbit muscle

Purification: The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Immunogen: L-Lactic dehydrogenase, LDH-5(M 4 ) isoenzyme, isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 6.7. No foreign proteins added.

Buffer: Biotin-coupled hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilised conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: Lactat Dehydrogenase (M4) (LDH-5)

Background: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.