RAB3A antibody (AA 202-217)

Catalog No. ABIN462094

This Rabbit Polyclonal antibody specifically detects RAB3A in WB, IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for RAB3A antibody (AA 202-217) (ABIN462094)

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RAB3A Antibody

Binding Specificity: AA 202-217

Brand: IHC-plus™

Specificity: Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

Purification: Immunoaffinity purified

Immunogen: A 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Platypus, Xenopus (88%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.2 (25  mM sodium borate, 100  mM boric acid, 75  mM NaCl, 5  mM EDTA (disodium salt)

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for RAB3A

Target: RAB3A (RAB3A, Member RAS Oncogene Family (RAB3A))

Alternative Name: RAB3A

Background: Name/Gene ID: RAB3A
Subfamily: RAS oncogene
Family: Ras GTPase superfamily IPR001806

Synonyms: RAB3A, RAS-associated protein RAB3A, Ras-related protein Rab-3A

Gene ID: 5864

UniProt: P20336

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport