NPC1 antibody

Catalog No. ABIN462125

The Mouse Monoclonal anti-NPC1 antibody has been validated for WB, IHC, IF, IP and IHC (p). It is suitable to detect NPC1 in samples from Human.

Quick Overview for NPC1 antibody (ABIN462125)

Target: NPC1 (Niemann-Pick Disease, Type C1 (NPC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NPC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NPC1 Antibody

Brand: IHC-plus™

Specificity: Reacts with the human NPC1 protein. Species cross-reactivity: Confirmed with an NPC1 transfected I1061T cell line.

Purification: Protein A purified

Immunogen: Fragments of Human NPC1 conjugated to a carrier protein.

Type of Immunogen: Protein

Isotype: IgG1

Application Details

Application Notes: Approved: IF (10 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NPC1

Target: NPC1 (Niemann-Pick Disease, Type C1 (NPC1))

Alternative Name: NPC / NPC1

Background: Name/Gene ID: NPC1

Synonyms: NPC1, NPC, Niemann-Pick C1 protein, Niemann-Pick disease, type C1

Gene ID: 4864