PRMT1 antibody (AA 317-346)

Catalog No. ABIN462306

Product Details anti-PRMT1 Antibody

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Binding Specificity: AA 317-346

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: This PRMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human PRMT1.

Purification: Ammonium sulfate precipitation

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Please note that cross-reactivity with mouse has been confirmed only in WB at this time. The antibody has not yet been evaluated for use in IHC with mouse samples.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Expiry Date: 6 months

Target Details for PRMT1

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Alternative Name: PRMT1 (PRMT1 Products)

Background: Name/Gene ID: PRMT1

Synonyms: PRMT1, HRMT1L2, HMT2, ANM1, HCP1, IR1B4

Gene ID: 3276

UniProt: Q99873