PRMT1 antibody (C-Term)

Catalog No. ABIN6264433

Product Details anti-PRMT1 Antibody

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: PRMT1 Antibody detects endogenous levels of total PRMT1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PRMT1, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PRMT1

Target: PRMT1 (Protein Arginine Methyltransferase 1 (PRMT1))

Alternative Name: PRMT1 (PRMT1 Products)

Synonyms: ANM1 antibody, HCP1 antibody, HRMT1L2 antibody, IR1B4 antibody, 6720434D09Rik antibody, AW214366 antibody, Hrmt1l2 antibody, Mrmt1 antibody, 3E10 antibody, anm1 antibody, hcp1 antibody, hrmt1l2 antibody, ir1b4 antibody, prmt1 antibody, prmt1b antibody, xPRMT1b antibody, xprmt1 antibody, xPRMT1 antibody, ARABIDOPSIS THALIANA PROTEIN ARGININE METHYLTRANSFERASE 1A antibody, ATPRMT1A antibody, F6F22.30 antibody, F6F22_30 antibody, protein arginine methyltransferase 1A antibody, DDBDRAFT_0183976 antibody, DDBDRAFT_0235399 antibody, DDB_0183976 antibody, DDB_0235399 antibody, PRMT1 antibody, DKFZp459J1326 antibody, fb39h07 antibody, wu:fb39h07 antibody, zf1 antibody, zgc:66201 antibody, protein arginine methyltransferase 1 antibody, protein arginine N-methyltransferase 1 antibody, protein arginine methyltransferase 1 L homeolog antibody, protein arginine methyltransferase 1 S homeolog antibody, protein arginine methyltransferase 1A antibody, protein arginine N-methyltransferase-1 antibody, protein arginine N-methyltransferase antibody, protein arginine methyltransferase antibody, PRMT1 antibody, Prmt1 antibody, prmt1.L antibody, prmt1.S antibody, PRMT1A antibody, prm-1 antibody, EDI_007800 antibody, Smp_029240.3 antibody, prmt1 antibody

Background:

Description: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4 and SERBP1 (PubMed:16879614). Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).

Gene: PRMT1

Molecular Weight: 42kDa

Gene ID: 3276

UniProt: Q99873