CD147 antibody

Catalog No. ABIN462349

This Rat Monoclonal antibody specifically detects CD147 in WB, FACS, IHC and IHC (p). It exhibits reactivity toward Mouse.

Quick Overview for CD147 antibody (ABIN462349)

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD147 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: OX114

Product Details anti-CD147 Antibody

Brand: IHC-plus™

Specificity: CD147 is a widely expressed membrane glycoprotein (also known as OX47, basigin, EMMPRIN and HT7) and has been implicated in matrix metalloproteinase induction, cell adhesion, retinal cell development and T cell activation. The transmembrane region has a v.

Purification: Protein G purified

Immunogen: Recombinant md0-2rCD4d3+4.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1 μg/10 x10⁶), IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Target Details for CD147

Target: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Alternative Name: Basigin / Emmprin / CD147

Background: Name/Gene ID: BSG

Synonyms: BSG, 5F7, Basigin, Basigin (Ok blood group), CD147 antigen, Collagenase stimulatory factor, EMMPRIN, OK, OK blood group antigen, TCSF, CD147, M6

Gene ID: 682

UniProt: P35613

Pathways: S100 Proteins