Interferon gamma antibody (Biotin)

Catalog No. ABIN465006

The Rabbit Polyclonal anti-Interferon gamma antibody has been validated for WB, ELISA and Neut. It is suitable to detect Interferon gamma in samples from Mouse.

Quick Overview for Interferon gamma antibody (Biotin) (ABIN465006)

Target: Interferon gamma (IFNG)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Interferon gamma Antibody (Biotin)

Specificity: Mouse IFN-gamma

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IFN-gamma.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Murine IFN-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IFN-gamma. To detect Murine IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IFN-gamma (LS-C104423) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IFN-gamma. Western Blot: To detect Murine IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN Gamma / Interferon Gamma

Background: Name/Gene ID: IFNG
Family: Interferon

Synonyms: IFNG, IFG, Immune interferon, IFI, Interferon gamma, IFN-gamma, Interferon, gamma

Gene ID: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy