CCL13 antibody (Biotin)

Catalog No. ABIN465155

This Goat Polyclonal antibody specifically detects CCL13 in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for CCL13 antibody (Biotin) (ABIN465155)

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL13 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL13 Antibody (Biotin)

Specificity: Human MCP-4

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human MCP-4.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hMCP-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-4. To detect hMCP-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human MCP-4 (LS-C104449) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMCP-4. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Alternative Name: CCL13 / MCP-4

Background: Name/Gene ID: CCL13
Family: Intercrine

Synonyms: CCL13, C-C motif chemokine 13, CKb10, MCP-4, MCP4, Monocyte chemotactic protein 4, New CC chemokine 1, Small-inducible cytokine A13, NCC1, CK-beta-10, NCC-1, SCYA13

Gene ID: 6357

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, The Global Phosphorylation Landscape of SARS-CoV-2 Infection