Ccl12 antibody (Biotin)

Catalog No. ABIN465850

This anti-Ccl12 antibody is a Rabbit Polyclonal antibody detecting Ccl12 in ELISA, WB and Neut. Suitable for Mouse.

Quick Overview for Ccl12 antibody (Biotin) (ABIN465850)

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ccl12 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Neutralization (Neut)

Product Details anti-Ccl12 Antibody (Biotin)

Specificity: Mouse MCP-5

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine MCP-5.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mMCP-5 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-5. To detect mMCP-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MCP-5 (LS-C104804) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-5. Western Blot: To detect mMCP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Ccl12

Target: Ccl12 (Chemokine (C-C Motif) Ligand 12 (Ccl12))

Alternative Name: CCL12 / MCP5

Background: Name/Gene ID: Ccl12

Synonyms: Ccl12, MCP-5, MCP5, Scya12

Gene ID: 20293

UniProt: Q62401

Pathways: Cellular Response to Molecule of Bacterial Origin