GRO gamma antibody

Catalog No. ABIN465908

The Rabbit Polyclonal anti-GRO gamma antibody has been validated for WB, ELISA and Neut. It is suitable to detect GRO gamma in samples from Human.

Quick Overview for GRO gamma antibody (ABIN465908)

Target: GRO gamma (Growth Regulated Oncogene gamma (GRO gamma))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRO gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-GRO gamma Antibody

Specificity: Human GROgamma

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human GROgamma.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (1 - 2 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 0.7 mg/mL-0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human GRO-gamma (100 ng/mL), a concentration of 1-2 μg/mL of this antibody is required. ELISA: To detect Human GRO-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GRO-gamma (LS-C104834) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human GRO-gamma. Western Blot: To detect Human GRO-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human GRO-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for GRO gamma

Target: GRO gamma (Growth Regulated Oncogene gamma (GRO gamma))

Alternative Name: CXCL3 / GRO Gamma

Background: Name/Gene ID: CXCL3
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL3, C-X-C motif chemokine 3, GRO3 oncogene, Growth-regulated protein gamma, GRO3, GRO-gamma, GRO-gamma(1-73), MGSA gamma, MIP2B, MIP-2b, MIP2-beta, CINC-2b, GROg, SCYB3, Small inducible cytokine b3

Gene ID: 2921

UniProt: P19876