PDGF-AA Homodimer antibody

Catalog No. ABIN7473215

This anti- antibody is a Goat Polyclonal antibody detecting in WB and Func. Suitable for Human, Cow, Pig and Rodents.

Quick Overview for PDGF-AA Homodimer antibody (ABIN7473215)

Target: PDGF-AA Homodimer

Reactivity: Human, Cow, Pig, Rodents

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Functional Studies (Func)

Product Details

Specificity: Recognizes non-reduced human PDGF AA, AB and BB dimers in western immunoblots. Porcine and bovine PDGF may be detected with decreased sensitivity. Rodent PDGF will not be detected. This antibody will neutralize human, porcine, bovine and rodent PDGF.

Purification: Ion exchange chromatography

Immunogen: Purified human PDGF-AB.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: Func, WB (5 - 10 μg/mL)

Usage: Suitable for use in Western Blot and Growth Inhibition Assays. 2. Prepare a 3T3 cell suspension at 10^4 cells/mL and aliquot 100 μL to each well. 3. Refeed on Day 3. 4. Count wells on Day 5 using an ATP endpoint assay. Wash cell layer once with PBS prior to lysis.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.0, 40 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: PDGF-AA Homodimer

Alternative Name: PDGF-AA

Background: Name/Gene ID: PDGFA
Family: PDGF

Synonyms: PDGFA, PDGF-A, PDGF A-chain, PDGF subunit A, PDGF-1, PDGF1

Gene ID: 5154

UniProt: P04085