Cyclin D1/D2 antibody

Catalog No. ABIN470767

This Mouse Monoclonal antibody specifically detects in IP, WB, IHC and IHC (p). It exhibits reactivity toward Human, Hamster, Mouse and Rat.

Quick Overview for Cyclin D1/D2 antibody (ABIN470767)

Target: Cyclin D1/D2

Reactivity: Human, Hamster, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Specificity: Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.

Purification: Protein A purified

Immunogen: Recombinant human Cyclin D1 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-P, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates cyclin D1 and D2 from 500 μg of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer,  pH 6.0, microwaved 3-5 minutes, three times).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Cyclin D1/D2

Alternative Name: Cyclin D1,2

Background: This antibody detects: Cyclin D1+D2