Cyclin D1/D2 antibody
Reactivity: Human, Hamster, Mouse, Rat
IP, WB, IHC, IHC (p)
Host: Mouse
Monoclonal
unconjugated
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- Target
- Cyclin D1/D2
- Reactivity
- Human, Hamster, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.
- Purification
- Protein A purified
- Immunogen
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Recombinant human Cyclin D1 expressed in E. coli.
Type of Immunogen: Recombinant protein - Isotype
- IgG2a
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- Application Notes
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Approved: IHC, IHC-P, IP, WB
Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates cyclin D1 and D2 from 500 μg of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer, pH 6.0, microwaved 3-5 minutes, three times). - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 50 % glycerol.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- Cyclin D1/D2
- Alternative Name
- Cyclin D1,2
- Background
- This antibody detects: Cyclin D1+D2
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