Cyclin D1/D2 antibody

Catalog No. ABIN470767

This Mouse Monoclonal antibody specifically detects not_set in IP, WB, IHC and IHC (p). It exhibits reactivity toward Human, Hamster, Mouse and Rat.

Quick Overview for Cyclin D1/D2 antibody (ABIN470767)

Target: Cyclin D1/D2

Reactivity: Human, Hamster, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details

Specificity: Recognizes p36 cyclin D1 and cross-reacts with p34 cyclin D2, which has a highly homologous epitope. No cross-reactivity with cyclin A, cyclin B or cyclin D3. Species cross-reactivity: human, mouse, rat and hamster.

Purification: Protein A purified

Immunogen: Recombinant human Cyclin D1 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-P, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5 μg immunoprecipitates cyclin D1 and D2 from 500 μg of A431 RIPA lysate. Immunohistochemistry: Reported to detect cyclin D1 and D2 in paraffin-embedded sections using high energy antigen retrieval (10 mM citrate buffer,  pH 6.0, microwaved 3-5 minutes, three times).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: Cyclin D1/D2

Alternative Name: Cyclin D1,2

Background: This antibody detects: Cyclin D1+D2