Soluble Terminal Complement Complex (sC5b-9) antibody

Catalog No. ABIN472099

This Mouse Monoclonal antibody specifically detects in FACS, IHC (fro), WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Soluble Terminal Complement Complex (sC5b-9) antibody (ABIN472099)

Target: Soluble Terminal Complement Complex (sC5b-9)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 3R2-0

Product Details

Specificity: Recognizes the SC5b-9 complex of around 330 kD. Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kD C5b which is bound to cells via 71 kD C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kD S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation. Removal of Sodium Azide is recommended prior to use in functional assays. Reported to work in western blotting applications.

Purification: Affinity purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Borate buffered saline,  pH 8.4, <0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details

Target: Soluble Terminal Complement Complex (sC5b-9)