MHC Class I (RT1a) antibody
Reactivity: Rat
FACS, IHC (fro), ELISA, IP, IHC
Host: Mouse
Monoclonal
OX-18
unconjugated
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- Target
- MHC Class I (RT1a)
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Reactivity
- Rat
-
Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
- Specificity
- Recognizes a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognized. MRC OX-18 has been used in immunoaffinity purification of rat MHC class I molecules. This product is routinely tested in flow cytometry on rat splenocytes.
- Purification
- Affinity purified
- Immunogen
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Rat spleen glycoproteins.
Type of Immunogen: Purified protein - Clone
- OX-18
- Isotype
- IgG1
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anti-MHC Class I (RT1a) antibody
Reactivity: Rat FACS, IHC (fro), ELISA, IP Host: Mouse Monoclonal OX-18 unconjugated
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- Application Notes
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Approved: ELISA, Flo (1:50 - 1:100), IHC, IHC-Fr, IP
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
-
Short term: -20°C
Long term: -20°C.
-
- Target
- MHC Class I (RT1a)
- Alternative Name
- MHC Class I RT1A
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