FZD6 antibody (Cysteine-Rich Domain, Extracellular)

Catalog No. ABIN478757

The Rat Monoclonal anti-FZD6 antibody has been validated for WB and ELISA. It is suitable to detect FZD6 in samples from Mouse.

Quick Overview for FZD6 antibody (Cysteine-Rich Domain, Extracellular) (ABIN478757)

Target: FZD6 (Frizzled Family Receptor 6 (FZD6))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FZD6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-FZD6 Antibody

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-6. Does not cross-react with rhFrizzled-5 or rmFrizzled-1, -2, -3, -4, -5, -7, -8 or -9.

Purification: Protein G purified

Immunogen: NS0-derived rmFrizzled-6 extracellular Cysteine-Rich Domain (CRD).

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Direct ELISA. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-6. Using a colorimetric detection system, the detection limit for rmFrizzled-6 is ~25 ng/lane under non-reducing conditions. Direct ELISA: Use at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-6. The detection limit for rmFrizzled-6 extracellular CRD is ~5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for FZD6

Target: FZD6 (Frizzled Family Receptor 6 (FZD6))

Alternative Name: FZD6 / Frizzled 6

Background: Name/Gene ID: FZD6
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD6, Frizzled family receptor 6, FZ-6, HFZ6, Frizzled 6, Frizzled-6, Frizzled homolog 6, FZ6, NDNC10

Gene ID: 8323

UniProt: O60353

Pathways: WNT Signaling, Tube Formation