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MHC Class II I-Ek antibody (Biotin)

The Mouse Monoclonal anti-MHC Class II I-Ek antibody has been validated for FACS, IHC, IP, CyTox and IHC (fro). It is suitable to detect MHC Class II I-Ek in samples from Mouse.
Catalog No. ABIN479336

Quick Overview for MHC Class II I-Ek antibody (Biotin) (ABIN479336)

Target

MHC Class II I-Ek

Reactivity

Mouse

Host

  • 12
Mouse

Clonality

  • 12
Monoclonal

Conjugate

  • 4
  • 3
  • 3
  • 2
This MHC Class II I-Ek antibody is conjugated to Biotin

Application

  • 11
  • 6
  • 6
  • 5
  • 5
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

14-4-4S
  • Specificity

    Recognizes an epitope on mouse I-Ek MHC class II alloantigen. Reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Has been reported to cross-react with the rat class II alloantigen RT1D.

    Purification

    Purified

    Immunogen

    C3H mouse skin graft and splenocytes.

    Type of Immunogen: Cells

    Isotype

    IgG2a kappa
  • Application Notes

    Approved: Ctox, Flo, IHC, IHC-Fr, IP

    Usage: Flow cytometry. Complement-dependent cytotoxicity. Immunoprecipitation. In vitro induction of antigen-specific B-cell differentiation. In vitro blocking of antigen presentation. Immunohistochemistry (acetone-fixed, frozen tissue sections). Flow Cytometry: 1 μg/1 x106. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS, sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target

    MHC Class II I-Ek
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