PCOLCE antibody (C-Term)

Catalog No. ABIN479694

This anti-PCOLCE antibody is a Goat Polyclonal antibody detecting PCOLCE in WB, IP and ELISA. Suitable for Mouse.

Quick Overview for PCOLCE antibody (C-Term) (ABIN479694)

Target: PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PCOLCE antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-PCOLCE Antibody

Binding Specificity: C-Term

Specificity: Selected for its ability to recognize mouse PCPE-1 in direct ELISAs and Western Blots. In these formats, this antibody shows ~35 % cross-reactivity with rhPCPE.

Purification: Immunoaffinity purified

Sterility: 0.2 μm filtered

Immunogen: NS0-derived rmPCPE-1.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IP, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse PCPE-1. The detection limit for rmPCPE-1 is approximately 1 ng/lane under non-reducing and reducing conditions. Immunoprecipitation: This antibody has been used to immunoprecipitate rmPCPE-1 from conditioned media of transfected NS0 cells. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse PCPE-1. The detection limit for rmPCPE-1 is approximately 0.5 ng/well.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute, sterile PBS, 40-50 % glycerol.

Concentration: Lot specific

Buffer: Lyophilized powder from a 0.2 um filtered solution in PBS, 5 % trehalose. Reconstitute, sterile PBS, 40-50 % glycerol.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized powder at 4°C. Reconstitute in PBS with 40-50% glycerol, aliquot and store at -20°C.

Target Details for PCOLCE

Target: PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))

Alternative Name: PCOLCE

Background: Name/Gene ID: PCOLCE

Synonyms: PCOLCE, PCPE, PCPE-1, PCPE1

Gene ID: 5118

UniProt: Q15113