PKC antibody (Ser660)

Catalog No. ABIN479946

The Rabbit Polyclonal anti-PKC antibody (ABIN479946) specifically detects PKC in WB, ELISA and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PKC antibody (Ser660) (ABIN479946)

Target: PKC (Protein Kinase C (PKC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-PKC Antibody

Binding Specificity: Ser660

Specificity: Detects endogenous levels of human PKC alpha, beta I, beta II, delta, epsilon and eta isoforms only when phosphorylated at a carboxy-terminal residue homologous to Ser660 of PKC beta II. Does not detect non-phosphorylated PKC or PKC phosphorylated at other sites. Apparent Molecular Weight: 78/80/82/85kD. Species cross-reactivity: Human, mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser660 of human PKC beta II.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:1000), IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Target Details for PKC

Target: PKC (Protein Kinase C (PKC))

Alternative Name: PKC / Protein Kinase C

Pathways: TCR Signaling, Regulation of G-Protein Coupled Receptor Protein Signaling, Interaction of EGFR with phospholipase C-gamma, Phototransduction