PKC antibody (Ser660)
Quick Overview for PKC antibody (Ser660) (ABIN479946)
Target
See all PKC AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- Ser660
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Specificity
- Detects endogenous levels of human PKC alpha, beta I, beta II, delta, epsilon and eta isoforms only when phosphorylated at a carboxy-terminal residue homologous to Ser660 of PKC beta II. Does not detect non-phosphorylated PKC or PKC phosphorylated at other sites. Apparent Molecular Weight: 78/80/82/85kD. Species cross-reactivity: Human, mouse and rat.
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Purification
- Immunoaffinity purified
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Immunogen
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Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser660 of human PKC beta II.
Type of Immunogen: Synthetic phospho-peptide -
Isotype
- IgG
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Application Notes
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Approved: ELISA (1:1000), IP (1:50), WB (1:1000)
Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:50. -
Comment
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
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Preservative
- Without preservative
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- PKC (Protein Kinase C (PKC))
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Alternative Name
- PKC / Protein Kinase C
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Pathways
- TCR Signaling, Regulation of G-Protein Coupled Receptor Protein Signaling, Interaction of EGFR with phospholipase C-gamma, Phototransduction
Target
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