Aconitase 1 antibody (C-Term, N-Term)

Catalog No. ABIN488534

The Rabbit Polyclonal anti-Aconitase 1 antibody has been validated for WB. It is suitable to detect Aconitase 1 in samples from Arabidopsis thaliana and Tomato. There is 1 publication available.

Quick Overview for Aconitase 1 antibody (C-Term, N-Term) (ABIN488534)

Target: Aconitase 1 (ACO1)

Reactivity: Arabidopsis thaliana, Tomato

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aconitase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Aconitase 1 Antibody

Binding Specificity: C-Term, N-Term

Cross-Reactivity (Details): Not reactive in: Chlamydomonas reinhardii

Predicted Reactivity: dicots including:Cucurbita maxima, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera,monocots: Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa

Characteristics: Expected / apparent Molecular Weight of the Antigene: 98 kDa. Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

Purification: serum

Immunogen: Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 - 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.

Application Details

Application Notes: 1:5 000 - 1:10 000 with standard ECL (WB). At higher concentrations the antibody binds aspecifically resulting in non-specific signals âper thousand 60 kDa, including Rubisco subunits.

Comment:

The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009). Possible differences in affinity have not been precisely quantified. Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure). Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-Aconitase 1 antibody (ABIN488534)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Target Details for Aconitase 1

Target: Aconitase 1 (ACO1)

Alternative Name: ACO1

Background: AGI Code: At4g35830
Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3

Molecular Weight: 98 kDa. Note that ACO1 kDa, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.

UniProt: Q42560

Pathways: Transition Metal Ion Homeostasis