AIRE antibody (AA 1-120)

Catalog No. ABIN4886444

The Rabbit Polyclonal anti-AIRE antibody has been validated for WB, IF, IHC, ICC and FACS. It is suitable to detect AIRE in samples from Human, Mouse and Rat.

Quick Overview for AIRE antibody (AA 1-120) (ABIN4886444)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-AIRE Antibody

Binding Specificity: AA 1-120

Purpose: Anti-AIRE Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AIRE Antibody Picoband® (ABIN4886444). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AIRE recombinant protein (Position: M1-K120). Human AIRE shares 87.2% amino acid (aa) sequence identity with mouse AIRE.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Lindmark E, Chen Y, Georgoudaki AM, Dudziak D, Lindh E, Adams WC, Loré K, Winqvist O, Chambers BJ, Karlsson MC, et al. (2013). "AIRE expressing marginal zone dendritic cells balances adaptive immunity and T-follicular helper cell recruitment.". Journal of Autoimmunity 42: 62-70. 2. Liston A, Lesage S, Wilson J, Peltonen L, Goodnow CC (April 2003). "Aire regulates negative selection of organ-specific T cells". Nat. Immunol. 4(4): 350-4.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE

Background:

Synonyms: Autoimmune regulator,Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein,APECED protein,AIRE,APECED,

Tissue Specificity: Widely expressed. Expressed at higher level in thymus (medullary epithelial cells and monocyte-dendritic cells), pancreas, adrenal cortex and testis. Expressed at lower level in the spleen, fetal liver and lymph nodes. Isoform 2 and isoform 3 seem to be less frequently expressed than isoform 1, if at all.

Background: The autoimmune regulator (AIRE) is a protein that in humans is encoded by the AIRE gene. This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The encoded protein plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED).

Molecular Weight: 58-68 kDa

Gene ID: 326

UniProt: O43918

Pathways: Chromatin Binding