CACYBP antibody (N-Term)

Catalog No. ABIN4886495

The Rabbit Polyclonal anti-CACYBP antibody has been validated for WB, IF, IHC, ICC, IP and FACS. It is suitable to detect CACYBP in samples from Human, Mouse and Rat.

Quick Overview for CACYBP antibody (N-Term) (ABIN4886495)

Target: CACYBP (Calcyclin Binding Protein (CACYBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CACYBP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-CACYBP Antibody

Binding Specificity: AA 7-47, N-Term

Purpose: Anti-SIAH Interacting Protein/CACYBP Antibody Picoband®

Sequence: QKDLEEVKVL LEKATRKRVR DALTAEKSKI ETEIKNKMQQ K

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-SIAH Interacting Protein/CACYBP Antibody (ABIN4886495). Tested in Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CACYBP, different from the related mouse sequence by five amino acids, and from the related rat sequence by six amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence,2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
1. Matsuzawa, S., Reed, J. C. Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses.Molec. Cell 7: 915-926, 2001. 2. Sun S, Ning X, Liu J, et al. (2007). "Overexpressed CacyBP/SIP leads to the suppression of growth in renal cell carcinoma.". Biochem. Biophys. Res. Commun. 356 (4): 864-71.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CACYBP

Target: CACYBP (Calcyclin Binding Protein (CACYBP))

Alternative Name: CACYBP

Background:

Synonyms: Calcyclin-binding protein,CacyBP,hCacyBP,S100A6-binding protein,Siah-interacting protein,CACYBP,S100A6BP, SIP,PNAS-107,

Tissue Specificity: Widely expressed.

Background: Calcyclin-binding protein is a protein that in humans is encoded by the CACYBP gene. And this gene is mapped to 1q24-q25. The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. In addition, it probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 26 kDa

Gene ID: 27101

Pathways: Response to Growth Hormone Stimulus