CCT3 antibody (C-Term)

Catalog No. ABIN4886513

The Rabbit Polyclonal anti-CCT3 antibody has been validated for WB, IHC, FACS, IF and ICC. It is suitable to detect CCT3 in samples from Human, Mouse and Rat.

Quick Overview for CCT3 antibody (C-Term) (ABIN4886513)

Target: CCT3 (Chaperonin Containing TCP1, Subunit 3 (Gamma) (CCT3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CCT3 Antibody

Binding Specificity: AA 497-536, C-Term

Purpose: Anti-CCT3 Antibody Picoband®

Sequence: EPLAVKLQTY KTAVETAVLL LRIDDIVSGH KKKGDDQSRQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CCT3 Antibody Picoband® (ABIN4886513). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CCT3, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: CCT3 chaperonin containing TCP1, subunit 3 (gamma)". 2. Joly EC, Sévigny G, Todorov IT, Bibor-Hardy V (Mar 1994). "cDNA encoding a novel TCP1-related protein".Biochimica et Biophysica Acta 1217 (2): 224-6. 3. Walkley NA, Demaine AG, Malik AN (Jan 1996)."Cloning, structure and mRNA expression of human Cctg, which encodes the chaperonin subunit CCT gamma". The Biochemical Journal 313 (Pt 2): 381-9.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCT3

Target: CCT3 (Chaperonin Containing TCP1, Subunit 3 (Gamma) (CCT3))

Alternative Name: CCT3

Background:

Synonyms: T-complex protein 1 subunit gamma,TCP-1-gamma,CCT-gamma,hTRiC5,CCT3,CCTG, TRIC5,

Tissue Specificity: Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Background: T-complex protein 1 subunit gamma is a protein that in humans is encoded by the CCT3 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8.

Molecular Weight: 61 kDa

Gene ID: 7203

UniProt: P49368