Complement Factor I antibody (AA 19-220)

Catalog No. ABIN4886537

The Rabbit Polyclonal anti-Complement Factor I antibody has been validated for WB, IHC, ICC and FACS. It is suitable to detect Complement Factor I in samples from Human and Rat.

Quick Overview for Complement Factor I antibody (AA 19-220) (ABIN4886537)

Target: Complement Factor I (CFI)

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Complement Factor I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Complement Factor I Antibody

Binding Specificity: AA 19-220

Purpose: Anti-Factor I/CFI Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Factor I/CFI Antibody Picoband® (ABIN4886537). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Factor I recombinant protein (Position: K19-D220). Human Factor I shares 70.7% and 71.2% amino acid (aa) sequence identity with mouse and rat Factor I, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: complement factor I". 2. Goldberger G, Bruns GA, Rits M, Edge MD, Kwiatkowski DJ (Jul 1987). "Human complement factor I: analysis of cDNA-derived primary structure and assignment of its gene to chromosome 4". The Journal of Biological Chemistry 262 (21): 10065-71.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Complement Factor I

Target: Complement Factor I (CFI)

Alternative Name: CFI

Background:

Synonyms: Complement factor I,3.4.21.45,C3B/C4B inactivator,Complement factor I heavy chain,Complement factor I light chain,CFI,IF,

Tissue Specificity: Plasma.

Background: Complement factor I, also known as C3b/C4b inactivator, is a protein that in humans is encoded by the CFI gene. This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene.

Molecular Weight: 75 kDa, 45 kDa

Gene ID: 3426

UniProt: P05156

Pathways: Complement System