HDEL antibody (C-Term)

Catalog No. ABIN488666

The Mouse Monoclonal anti-HDEL antibody has been validated for WB, IF and EM. It is suitable to detect HDEL in samples from Saccharomyces cerevisiae, Drosophila melanogaster and Barnyard Grass (Echinochloa crus-galli).

Quick Overview for HDEL antibody (C-Term) (ABIN488666)

Target: HDEL

Reactivity: Saccharomyces cerevisiae, Drosophila melanogaster, Barnyard Grass (Echinochloa crus-galli)

Host: Mouse

Clonality: Monoclonal

Conjugate: This HDEL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Electron Microscopy (EM)

Clone: 2E7

Product Details anti-HDEL Antibody

Binding Specificity: C-Term

Predicted Reactivity: not analyzed

Characteristics: Expected / apparent Molecular Weight of the Antigene: 78 kDa

Purification: affinity purified

Immunogen: synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

Isotype: IgG2b

Application Details

Application Notes: Recommended Dilution: 1 : 100 - 1. 1000 (WB), 1. 50- 1 : 500 (IF)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Make aliquots to avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year.

Expiry Date: 12 months

Target Details for HDEL

Target: HDEL

Alternative Name: HDEL

Background: HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

Molecular Weight: 78 kDa