HDEL antibody (C-Term)

Catalog No. ABIN488666

The Mouse Monoclonal anti-HDEL antibody is suitable to detect HDEL in samples from Saccharomyces cerevisiae, Drosophila melanogaster, Beet, Cotton, Mung bean, Barley, Echinochloa crus-galli and Sorghum. It has been validated for WB, IF and EM.

Quick Overview for HDEL antibody (C-Term) (ABIN488666)

Target: HDEL

Reactivity: Saccharomyces cerevisiae, Drosophila melanogaster, Beet, Cotton, Mung bean, Barley, Echinochloa crus-galli, Sorghum

Host: Mouse

Clonality: Monoclonal

Conjugate: This HDEL antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Electron Microscopy (EM)

Clone: 2E7

Product Details anti-HDEL Antibody

Binding Specificity: C-Term

Purpose: Anti-HDEL | Endoplasmic reticulum retention signal (clone 2E7)

Cross-Reactivity (Details): Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Higher plants

Purification: Protein G purified

Immunogen: Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein

Isotype: IgG2b

Application Details

Application Notes: 1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Total IgG. Protein G purified in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store at -20°C for 1 year, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Expiry Date: 12 months

Target Details for HDEL

Target: HDEL

Alternative Name: HDEL

Background:

Background: HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.

Additional information: Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/mL

Molecular Weight: 78 kDa