HDEL antibody (C-Term)
HDEL
Reactivity: Saccharomyces cerevisiae, Drosophila melanogaster, Barnyard Grass (Echinochloa crus-galli)
WB, IF, EM
Host: Mouse
Monoclonal
2E7
unconjugated
-
- Target See all HDEL products
- HDEL
- Binding Specificity
- C-Term
-
Reactivity
- Saccharomyces cerevisiae, Drosophila melanogaster, Barnyard Grass (Echinochloa crus-galli)
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Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This HDEL antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Electron Microscopy (EM)
- Predicted Reactivity
- not analyzed
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 78 kDa
- Purification
- affinity purified
- Immunogen
- synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
- Clone
- 2E7
- Isotype
- IgG2b
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-
- Application Notes
- Recommended Dilution: 1 : 100 - 1. 1000 (WB), 1. 50- 1 : 500 (IF)
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Make aliquots to avoid repeated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- Store at -20 °C for 1 year.
- Expiry Date
- 12 months
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- Target
- HDEL
- Alternative Name
- HDEL (HDEL Products)
- Background
- HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
- Molecular Weight
- 78 kDa
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