MPI antibody (AA 2-99)

Catalog No. ABIN4886672

The Rabbit Polyclonal anti-MPI antibody has been validated for WB, IHC and FACS. It is suitable to detect MPI in samples from Human, Rat and Mouse.

Quick Overview for MPI antibody (AA 2-99) (ABIN4886672)

Target: MPI (Mannose Phosphate Isomerase (MPI))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPI antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MPI Antibody

Binding Specificity: AA 2-99

Purpose: Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Mannose Phosphate Isomerase/MPI Antibody Picoband® (ABIN4886672). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Jaeken, J., Matthijs, G., Saudubray, J.-M., Dionisi-Vici, C., Bertini, E., de Lonlay, P., Henri, H., Carchon, H., Schollen, E., Van Schaftingen, E. Phosphomannose isomerase deficiency: a carbohydrate-deficient glycoprotein syndrome with hepatic-intestinal presentation. (Letter) Am. J. Hum. Genet. 62: 1535-1539, 1998. 2. Schollen, E., Dorland, L., de Koning, T. J., Van Diggelen, O. P., Huijmans, J. G. M., Marquardt, T., Babovic-Vuksanovic, D., Patterson, M., Imtiaz, F., Winchester, B., Adamowicz, M., Pronicka, E., Freeze, H., Matthijs, G. Genomic organization of the human phosphomannose isomerase (MPI) gene and mutation analysis in patients with congenital disorders of glycosylation type Ib (CDG-Ib). Hum. Mutat. 16: 247-252, 2000.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MPI

Target: MPI (Mannose Phosphate Isomerase (MPI))

Alternative Name: MPI

Background:

Synonyms: Mannose-6-phosphate isomerase,5.3.1.8,Phosphohexomutase,Phosphomannose isomerase,PMI,MPI,PMI1,

Tissue Specificity: Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.

Background: Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate (F6P) and mannose-6-phosphate (M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.

Molecular Weight: 47 kDa

Gene ID: 4351

UniProt: P34949