PVRL4 antibody (N-Term)

Catalog No. ABIN4886678

The Rabbit Polyclonal anti-PVRL4 antibody has been validated for WB. It is suitable to detect PVRL4 in samples from Human.

Quick Overview for PVRL4 antibody (N-Term) (ABIN4886678)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PVRL4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PVRL4 Antibody

Binding Specificity: AA 53-94, N-Term

Purpose: Anti-PVRL4/NECTIN4 Antibody Picoband®

Sequence: FYRGDSGEQV GQVAWARVDA GEGAQELALL HSKYGL

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PVRL4/NECTIN4 Antibody Picoband® (ABIN4886678). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nectin-4/PVRL4, different from the related mouse sequence by seven amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Brancati, F., Fortugno, P., Bottillo, I., Lopez, M., Josselin, E., Boudghene-Stambouli, O., Agolini, E., Bernardini, L., Bellacchio, E., Iannicelli, M., Rossi, A., Dib-Lachachi, A., Stuppia, L., Palka, G., Mundlos, S., Stricker, S., Kornak, U., Zambruno, G., Dallapiccola, B.Mutations in PVRL4, encoding cell adhesion molecule nectin-4, cause ectodermal dysplasia-syndactyly syndrome.Am. J. Hum. Genet. 87: 265-273, 2010. 2. Fabre-Lafay, S., Garrido-Urbani, S., Reymond, N., Goncalves, A., Dubreuil, P., Lopez, M. Nectin-4, a new serological breast cancer marker, is a substrate for tumor necrosis factor-alpha-converting enzyme (TACE)/ADAM-17. J. Biol. Chem. 280: 19543-19550, 2005. 3. Muhlebach, M. D., Mateo, M., Sinn, P. L., Prufer, S., Uhlig, K. M., Leonard, V. H. J., Navaratnarajah, C. K., Frenzke, M., Wong, X. X., Sawatsky, B., Ramachandran, S., McCray, P. B., Jr., Cichutek, K., von Messling, V., Lopez, M., Cattaneo, R. Adherens junction protein nectin-4 is the epithelial receptor for measles virus. Nature 480: 530-533, 2011.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PVRL4

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: NECTIN4

Background:

Synonyms: Nectin-4,Ig superfamily receptor LNIR,Nectin cell adhesion molecule 4 ,Poliovirus receptor-related protein 4,Processed poliovirus receptor-related protein 4,NECTIN4 ,LNIR, PRR4, PVRL4,

Tissue Specificity: Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. .

Background: PVRL4, also known as Nectin-4, is expressed in human skin, hair follicles, and cultured keratinocytes, but not in fibroblasts. This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.

Molecular Weight: 66 kDa

Gene ID: 81607

Pathways: Cell-Cell Junction Organization