PDCD1LG2 antibody (AA 20-220)

Catalog No. ABIN4886690

The Rabbit Polyclonal anti-PDCD1LG2 antibody has been validated for WB. It is suitable to detect PDCD1LG2 in samples from Human.

Quick Overview for PDCD1LG2 antibody (AA 20-220) (ABIN4886690)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCD1LG2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDCD1LG2 Antibody

Binding Specificity: AA 20-220

Purpose: Anti-B7 DC/PDCD1LG2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-B7 DC/PDCD1LG2 Antibody Picoband® (ABIN4886690). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human B7 DC recombinant protein (Position: L20-T220). Human B7 DC shares 72.1% amino acid (aa) sequence identity with mouse B7 DC.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: PDCD1LG2 programmed cell death 1 ligand 2". 2. Latchman Y, Wood CR, Chernova T, Chaudhary D, Borde M, Chernova I, Iwai Y, Long AJ, Brown JA, Nunes R, Greenfield EA, Bourque K, Boussiotis VA, Carter LL, Carreno BM, Malenkovich N, Nishimura H, Okazaki T, Honjo T, Sharpe AH, Freeman GJ (Mar 2001). "PD-L2 is a second ligand for PD-1 and inhibits T cell activation". Nat Immunol 2 (3): 261-8. 3. Zhang, Y., Chung, Y., Bishop, C., Daugherty, B., Chute, H., Holst, P., Kurahara, C., Lott, F., Sun, N., Welcher, A. A., Dong, C.Regulation of T cell activation and tolerance by PDL2. Proc. Nat. Acad. Sci. 103: 11695-11700, 2006.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PDCD1LG2

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: PDCD1LG2

Background:

Synonyms: Programmed cell death 1 ligand 2,PD-1 ligand 2,PD-L2,PDCD1 ligand 2,Programmed death ligand 2,Butyrophilin B7-DC,B7-DC,CD273,PDCD1LG2,B7DC, CD273, PDCD1L2, PDL2,

Tissue Specificity: Highly expressed in heart, placenta, pancreas, lung and liver and weakly expressed in spleen, lymph nodes and thymus. .

Background: Programmed cell death 1 ligand 2 (also known as PD-L2, B7-DC) is a protein that in humans is encoded by the PDCD1LG2 gene. It is mapped to chromosome 9p24.2. PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. Northern blot analysis detect wide expression of PDL2, with highest levels in placenta, heart, pancreas, lung, and liver, and lower levels in spleen, lymph nodes, and thymus. PDL2 negatively regulates T cells and plays an essential role in immune tolerance.

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molecular Weight: 37 kDa

Gene ID: 80380