PSMA4 antibody (Middle Region)

Catalog No. ABIN4886707

This Rabbit Polyclonal antibody specifically detects PSMA4 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PSMA4 antibody (Middle Region) (ABIN4886707)

Target: PSMA4 (Proteasome Subunit alpha 4 (PSMA4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMA4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PSMA4 Antibody

Binding Specificity: AA 84-123, Middle Region

Purpose: Anti-PSMA4 Antibody Picoband®

Sequence: NVLTNELRLI AQRYLLQYQE PIPCEQLVTA LCDIKQAYTQ

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PSMA4 Antibody Picoband® (ABIN4886707). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PSMA4, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Groll M, Ditzel L, Löwe J, Stock D, Bochtler M, Bartunik HD, Huber R (Apr 1997). "Structure of 20S proteasome from yeast at 2.4 A resolution". Nature 386 (6624): 463-71. 2. Tomko RJ, Hochstrasser M (2013). "Molecular architecture and assembly of the eukaryotic proteasome". Annual Review of Biochemistry 82: 415-45.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PSMA4

Target: PSMA4 (Proteasome Subunit alpha 4 (PSMA4))

Alternative Name: PSMA4

Background:

Synonyms: Proteasome subunit alpha type-4,3.4.25.1,Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit L,PSMA4,HC9, PSC9,

Background: Proteasome subunit alpha type-4, also known as macropain subunit C9, proteasome component C9, and 20S proteasome subunit alpha-3, is a protein that in humans is encoded by the PSMA4 gene. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 29 kDa

Gene ID: 5685

UniProt: P25789

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA