RPS27 antibody (AA 2-84)

Catalog No. ABIN4886714

The Rabbit Polyclonal anti-RPS27 antibody has been validated for WB, IHC, IF, FACS, ICC and IHC (fro). It is suitable to detect RPS27 in samples from Human, Mouse and Rat.

Quick Overview for RPS27 antibody (AA 2-84) (ABIN4886714)

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-RPS27 Antibody

Binding Specificity: AA 2-84

Purpose: Anti-MPS1/RPS27 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MPS1/RPS27 Antibody Picoband® (ABIN4886714). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human MPS1 recombinant protein (Position: P2-H84). Human MPS1 shares 100% amino acid (aa) sequence identity with both mouse and rat MPS1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Fernandez-Pol JA (1996). "Metallopanstimulin as a novel tumor marker in sera of patients with various types of common cancers: implications for prevention and therapy". Anticancer Research 16 (4B): 2177-85. 2. Tsui SK, Lee SM, Fung KP, Waye MM, Lee CY (Oct 1996). "Primary structures and sequence analysis of human ribosomal proteins L39 and S27". Biochemistry and Molecular Biology International 40 (3): 611-6.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RPS27

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Alternative Name: RPS27

Background:

Synonyms: 40S ribosomal protein S27,Metallopan-stimulin 1,MPS-1,RPS27,MPS1,

Tissue Specificity: Expressed in a wide variety of actively proliferating cells and tumor tissues.

Background: 40S ribosomal protein S27, also known as metallopan-stimulin 1 or MPS-1, is a protein that in humans is encoded by the RPS27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Molecular Weight: 10 kDa

Gene ID: 6232

UniProt: P42677