SLC2A5 antibody (Middle Region)

Catalog No. ABIN4886727

This anti-SLC2A5 antibody is a Rabbit Polyclonal antibody detecting SLC2A5 in WB, IHC, IF, FACS, IHC (fro) and ICC. Suitable for Human and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for SLC2A5 antibody (Middle Region) (ABIN4886727)

Target: SLC2A5 (Solute Carrier Family 2 (Facilitated Glucose/fructose Transporter), Member 5 (SLC2A5))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC2A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-SLC2A5 Antibody

Binding Specificity: AA 223-259, Middle Region

Purpose: Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband®

Sequence: QKKDEAAAKK ALQTLRGWDS VDREVAEIRQ EDEAEKA

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Glucose Transporter 5 GLUT5/SLC2A5 Antibody Picoband® (ABIN4886727). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SLC2A5, different from the related mouse and rat sequences by nine amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Burant, C. F., Takeda, J., Brot-Laroche, E., Bell, G. I., Davidson, N. O.Fructose transporter in human spermatozoa and small intestine is GLUT5.J. Biol. Chem. 267: 14523-14526, 1992. 2. Davidson, N. O., Hausman, A. M. L., Ifkovits, C. A., Buse, J. B., Gould, G. W., Burant, C. F., Bell, G. I.Human intestinal glucose transporter expression and localization of GLUT5.Am. J. Physiol. 262: C795-C800, 1992. 3. Kayano, T., Burant, C. F., Fukumoto, H., Gould, G. W., Fan, Y. S., Eddy, R. L., Byers, M. G., Shows, T. B., Seino, S., Bell, G. I.Human facilitative glucose transporters: isolation, functional characterization, and gene localization of cDNAs encoding an isoform (GLUT5) expressed in small intestine, kidney, muscle, and adipose tissue and an unusual glucose transporter pseudogene-like sequence (GLUT6).J. Biol. Chem. 265: 13276-13282, 1990.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-SLC2A5 antibody (ABIN4886727)

Li, Byrd, Doh, Dixon, Lee, Tiwari, Ecelbarger: "Absence of renal enlargement in fructose-fed proximal-tubule-select insulin receptor (IR), insulin-like-growth factor receptor (IGF1R) double knockout mice." in: Physiological reports, Vol. 4, Issue 23, (2018) (PubMed).

Target Details for SLC2A5

Target: SLC2A5 (Solute Carrier Family 2 (Facilitated Glucose/fructose Transporter), Member 5 (SLC2A5))

Alternative Name: SLC2A5

Background:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 5,Fructose transporter,Glucose transporter type 5, small intestine,GLUT-5,SLC2A5,GLUT5,

Tissue Specificity: Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.

Background: SLC2A5, also known as GLUT5 (Glucose transporter 5), is a fructose transporter expressed on the apical border of enterocytes in the small intestine. The GLUT5 gene is located on chromosome 1. GLUT5 allows for fructose to be transported from the intestinal lumen into the enterocyte by facilitated diffusion due to fructose's high concentration in the intestinal lumen. GLUT5 is also expressed in skeletal muscle, testis, kidney, fat tissue, and brain. Fructose malabsorption or Dietary Fructose Intolerance is a dietary disability of the small intestine, where the amount of fructose carrier in enterocytes is deficient.

Molecular Weight: 55 kDa

Gene ID: 6518

UniProt: P22732