TLN2 antibody (Middle Region)

Catalog No. ABIN4886745

Product Details anti-TLN2 Antibody

Target: TLN2 (Talin 2 (TLN2))

Binding Specificity: AA 1787-1822, Middle Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLN2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Purpose: Anti-Talin 2/TLN2 Antibody Picoband®

Sequence: NPKAQHTHDA ITEAAQLMKE AVDDIMVTLN EAASEV

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Talin 2/TLN2 Antibody Picoband® (ABIN4886745). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Talin 2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: Talin 2". 2. Monkley SJ, Pritchard CA, Critchley DR (Sep 2001). "Analysis of the mammalian talin2 gene TLN2". Biochemical and Biophysical Research Communications 286 (5): 880-5. 3. Praekelt U, Kopp PM, Rehm K, Linder S, Bate N, Patel B, Debrand E, Manso AM, Ross RS, Conti F, Zhang MZ, Harris RC, Zent R, Critchley DR, Monkley SJ (Mar 2012). "New isoform-specific monoclonal antibodies reveal different sub-cellular localisations for talin1 and talin2". European Journal of Cell Biology 91 (3): 180-91.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-TLN2 antibody (ABIN4886745)

Li, Li, Qi, Rychahou, Jafari, Huang: "The role of talin2 in breast cancer tumorigenesis and metastasis." in: Oncotarget, Vol. 8, Issue 63, pp. 106876-106887, (2017) (PubMed).

Target Details for TLN2

Target: TLN2 (Talin 2 (TLN2))

Alternative Name: TLN2 (TLN2 Products)

Background:

Synonyms: Talin-2,TLN2,KIAA0320,

Tissue Specificity: Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.

Background: Talin 2 is a protein in humans that is encoded by the TLN2 gene. It belongs to the talin protein family. This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. Talin-2 is expressed at high levels in cardiac muscle and functions to provide linkages between the extracellular matrix and actin cytoskeleton at costamere structures to transduce force laterally.

Molecular Weight: 271 kDa

Gene ID: 83660

UniProt: Q9Y4G6

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location