ZBTB16 antibody (AA 1-165)

Catalog No. ABIN4886774

The Rabbit Polyclonal anti-ZBTB16 antibody has been validated for WB. It is suitable to detect ZBTB16 in samples from Human and Rat.

Quick Overview for ZBTB16 antibody (AA 1-165) (ABIN4886774)

Target: ZBTB16 (Zinc Finger and BTB Domain Containing 16 (ZBTB16))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZBTB16 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ZBTB16 Antibody

Binding Specificity: AA 1-165

Purpose: Anti-Plzf/ZBTB16 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Plzf/ZBTB16 Antibody Picoband® (ABIN4886774). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Plzf recombinant protein (Position: M1-E165).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Kovalovsky D, Uche OU, Eladad S, Hobbs RM, Yi W, Alonzo E, Chua K, Eidson M, et al. (2008). "The BTB-zinc finger transcriptional regulator, PLZF, controls the development of iNKT cell effector functions". Nature Immunology 9 (9): 1055-64. 2. Savage AK, Constantinides MG, Han J, Picard D, Martin E, Li B, Lantz O, Bendelac A (2008). "The transcription factor PLZF (Zbtb16) s the effector program of the NKT cell lineage".Immunity 29 (3): 391-403. 3. Zhang T, Xiong H, Kan LX, Zhang CK, Jiao XF, Fu G, Zhang QH, Lu L, et al. (1999). "Genomic sequence, structural organization, molecular evolution, and aberrant rearrangement of promyelocytic leukemia zinc finger gene".Proceedings of the National Academy of Sciences of the United States of America 96 (20): 11422-7.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ZBTB16

Target: ZBTB16 (Zinc Finger and BTB Domain Containing 16 (ZBTB16))

Alternative Name: ZBTB16

Background:

Synonyms: Zinc finger and BTB domain-containing protein 16,Promyelocytic leukemia zinc finger protein,Zinc finger protein 145,Zinc finger protein PLZF,ZBTB16,PLZF, ZNF145,

Tissue Specificity: Within the hematopoietic system, PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. Also expressed in the ovary, and at lower levels, in the kidney and lung.

Background: Zinc finger and BTB domain-containing protein 16 is a protein that in humans is encoded by the ZBTB16 gene. This gene is a member of the Krueppel C2H2-type zinc-finger protein family and encodes a zinc finger transcription factor that contains nine Kruppel-type zinc finger domains at the carboxyl terminus. This protein is located in the nucleus, is involved in cell cycle progression, and interacts with a histone deacetylase. Specific instances of aberrant gene rearrangement at this locus have been associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been characterized.

Molecular Weight: 74 kDa

Gene ID: 7704

UniProt: Q05516

Pathways: Positive Regulation of fat Cell Differentiation