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Histone 3 antibody (H3K27me2, H3K27me3)

This Mouse Monoclonal antibody specifically detects Histone 3 in WB, IF and ICC. It exhibits reactivity toward Human and has been mentioned in 4+ publications.
Catalog No. ABIN4889658

Quick Overview for Histone 3 antibody (H3K27me2, H3K27me3) (ABIN4889658)

Target

See all Histone 3 (H3) Antibodies
Histone 3 (H3) (Histone H3 (H3))

Reactivity

  • 1344
  • 909
  • 811
  • 41
  • 39
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  • 26
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  • 6
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  • 1
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Host

  • 1157
  • 250
  • 8
  • 5
Mouse

Clonality

  • 1075
  • 344
  • 1
Monoclonal

Conjugate

  • 814
  • 72
  • 44
  • 44
  • 44
  • 44
  • 44
  • 44
  • 29
  • 27
  • 18
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  • 1
This Histone 3 antibody is un-conjugated

Application

  • 1028
  • 417
  • 374
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  • 202
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  • 3
  • 1
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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone

7B11
  • Binding Specificity

    • 52
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    • 23
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    • 8
    H3K27me2, H3K27me3

    Purification

    Protein A Chromatography

    Immunogen

    This Histone H3 di/trimethylLys27 antibody was raised against a peptide including trimethyllysine 27 of histone H3.

    Isotype

    IgG
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 μg/μL

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

    Storage

    -20 °C

    Storage Comment

    Antibodies in solution can be stored at -20 °C for 2 years.

    Expiry Date

    6 months
  • Nolan, Franco, Hance, Hayward, Isaacs: "Tumor-secreted Hsp90 subverts polycomb function to drive prostate tumor growth and invasion." in: The Journal of biological chemistry, Vol. 290, Issue 13, pp. 8271-82, (2015) (PubMed).

    Papazyan, Voronina, Chapman, Luperchio, Gilbert, Meier, Mackintosh, Shabanowitz, Tackett, Reddy, Coyne, Hunt, Liu, Taverna: "Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis." in: eLife, Vol. 3, pp. e02996, (2014) (PubMed).

    Ivanov, Pawlikowski, Manoharan, van Tuyn, Nelson, Rai, Shah, Hewitt, Korolchuk, Passos, Wu, Berger, Adams: "Lysosome-mediated processing of chromatin in senescence." in: The Journal of cell biology, Vol. 202, Issue 1, pp. 129-43, (2013) (PubMed).

    Rada-Iglesias, Bajpai, Prescott, Brugmann, Swigut, Wysocka: "Epigenomic annotation of enhancers predicts transcriptional regulators of human neural crest." in: Cell stem cell, Vol. 11, Issue 5, pp. 633-48, (2012) (PubMed).

  • Target

    Histone 3 (H3) (Histone H3 (H3))

    Alternative Name

    Histone H3

    Molecular Weight

    17 kDa

    Gene ID

    3020
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