CD3 epsilon antibody

Catalog No. ABIN490320

This anti-CD3 epsilon antibody is a Armenian Hamster Monoclonal antibody detecting CD3 epsilon in FACS, IHC, IP, Func, IHC (fro), ICC and Dep. Suitable for Mouse.

Quick Overview for CD3 epsilon antibody (ABIN490320)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Depletion (Dep)

Clone: 145-2C11

Product Details anti-CD3 epsilon Antibody

Specificity: The Armenian hamster monoclonal antibody 145-2C11 reacts with mouse CD3 (epsilon subunit). This antibody is commonly used as a phenotypic marker for mouse T cells.

Purification: Protein A purified

Endotoxin Level: Low Endotoxin

Immunogen: Mouse BM10-37 cytotoxic T lymphocytes.

Isotype: IgG

Application Details

Application Notes: Approved: Depl, Flo, Func, ICC, IHC, IHC-Fr, IP

Usage: Flow Cytometry: Recommended dilution - 1-2 μg / ml (million cells). Immunoprecipitation: Recommended dilution - 1-2 μg / 100-500 μg protein in 1 mL cell lysate. Functional Application: Induction of T cell activation, proliferation or apoptosis (depending The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Azide-free PBS,  pH 7.4, 0.2 micron filter sterilized

Preservative: Azide free

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background: Name/Gene ID: CD3E

Synonyms: CD3E, CD3e antigen, CD3-epsilon, T-cell receptor epsilon chain, TCRE, T-cell receptors epsilon chain, T3E, CD3 Epsilon

Gene ID: 916

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway