Hemoglobin Subunit beta antibody

Catalog No. ABIN4903870

This Rabbit Polyclonal antibody specifically detects Hemoglobin Subunit beta in WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Hemoglobin Subunit beta antibody (ABIN4903870)

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hemoglobin Subunit beta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Hemoglobin Subunit beta Antibody

Characteristics: Hemoglobin (Hgb) is coupled to four iron-binding, methene-linked tetrapyrrole rings (heme). The å (16p13.3, 5'-Ω-pseudoz-pseudo å2-pseudo å1-å2-å1-1-3') and (11p15.5) globin loci determine the basic hemoglobin structure. The globin portion of hemoglobin consists of two å chains and two chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between å and chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the å1-2 cleavage plane. When the two å1-2 interfaces are closely bound, hemoglobin has a low affinity for oxygen. Hb A, which contains two å chains plus two chains, comprises 97 % of total circulating hemoglobin. The remaining 3 % of total circulating hemoglobin is comprised of Hb A-2, which consists of two å chains plus two chains, and fetal hemoglobin (Hb F), which consists of two å chains together with two © chains.

Purification: Affinity purification

Immunogen: Recombinant protein of human HBB.

Isotype: IgG

Application Details

Application Notes: WB 1:100-1:200 , IHC 1:50-1:100 , FC 1:20-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for Hemoglobin Subunit beta

Target: Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))

Alternative Name: HBB

Gene ID: 3043

UniProt: P68871